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M1302

Sigma-Aldrich

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Sinónimos:

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

Número de CAS:
9068-38-6
Número MDL:
MFCD00283754
Código UNSPSC:
12352200
NACRES:
NA.55

origen biológico

Porcine intestinal mucosa

Nivel de calidad

200

recombinante

expressed in E. coli

formulario

liquid

uso

sufficient for 200 reactions
 sufficient for 250 reactions

Características

dNTPs included: no
hotstart: no

concentración

200 units/μL

técnicas

RT-PCR: suitable

color

colorless

entrada

purified RNA

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Aplicación

M-MLV Reverse Transcriptase has been used:
  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • in quantitative realtime-polymerase chain reaction (RT-qPCR)
  • in reverse transcription

Características y beneficios

  • Thermostable reverse transcriptase active at 37 °C.
  • Can be used to generate first strand cDNA of up to 7 kb.

Envase

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Definición de unidad

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Nota de preparación

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Información legal

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

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Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
G F Gerard et al.
DNA (Mary Ann Liebert, Inc.), 5(4), 271-279 (1986-08-01)
We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region
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Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

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