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L2879

Sigma-Aldrich

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

Sinónimos:

Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

Número de CAS:
9066-59-5
Comisión internacional de enzimas:
3.2.1.17 (BRENDA, IUBMB)
Número CE:
232-954-0
Número MDL:
MFCD00131557
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

chicken egg white

tpo

Grade VI

formulario

lyophilized powder

actividad específica

≥35,000 units/mg protein (E1%/282)

mol peso

~14.3 kDa

composición

Protein, ≥80%

técnicas

cell based assay: suitable

idoneidad

suitable for cell lysis

aplicaciones

cell analysis

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Aplicación

Lysozyme chloride form from chicken egg white has been used:
  • as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
  • in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
  • in viscometric assay for determining lysozyme activity    

Acciones bioquímicas o fisiológicas

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Definición de unidad

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Forma física

Lyophilized powder containing sodium chloride and sodium acetate

Nota de preparación

3× Crystallized

sustrato

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Journal of dairy science, 91(5), 1759-1768 (2008-04-19)
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The journal of physical chemistry. B, 117(4), 1186-1195 (2013-01-11)
High-resolution inelastic X-ray scattering was used to investigate the collective vibrational excitations in hydrated lysozyme powders as a function of hydration level and temperature. It is found that the samples with strong enzymatic function are "soft", in the sense that
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Acta crystallographica. Section D, Biological crystallography, 69(Pt 2), 284-297 (2013-02-07)
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Artículos

Carbohydrate Analysis III

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolos

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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