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KCQS05

Sigma-Aldrich

KiCqStart® Probe qPCR ReadyMix™, Low ROX™

with Low ROX for ABI and Stratagene instruments

Sinónimos:

Kicqstart® Probe qPCR ReadyMix™

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

formulario

liquid

uso

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Características

dNTPs included
hotstart

condiciones de almacenamiento

protect from light

técnicas

qPCR: suitable

color

light blue

entrada

purified DNA

compatibilidad

ABI 7500 Fast
 ABI 7500
 ABI QuantStudio
 ABI ViiA 7
Agilent AriaMx
 Douglas Scientific IntelliQube
 Qiagen Corbett Rotor-Gene Q
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

método de detección

probe-based

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Descripción general

KiCqStart® Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Aplicación

KiCqStart® Probe qPCR ReadyMix™, Low ROX™ has been used as a component of reaction mixture for detection of genomic DNA extracted from parasites by multiplex quantitative polymerase chain reaction (qPCR) and Real-Time PCR (qPCR).
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Características y beneficios

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Componentes

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Información legal

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Surveillance of berries sold on the Norwegian market for parasite contamination using molecular methods
Temesgen TT, et al.
Food Microbiology, 103980-103980 (2022)
Alessandra Barlaam et al.
Food microbiology, 98, 103792-103792 (2021-04-21)
To investigate the presence of Cyclospora cayetanensis, Toxoplasma gondii and Echinococcus spp. in fresh produce sold in Italy, 324 locally produced 'ready-to-eat' (RTE) mixed-salad packages belonging to three brands and 324 berries packages (blueberries and blackberries imported from Peru and
Tamirat T Temesgen et al.
Foods (Basel, Switzerland), 10(2) (2021-03-07)
Due to the delicate nature of berries and the reduced shelf-life once washed, producers usually do not wash berries. Therefore, consumers are expected to wash the berries prior to consumption, and this might be a more effective way of infection
Tamirat Tefera Temesgen et al.
Food research international (Ottawa, Ont.), 125, 108636-108636 (2019-09-27)
Foodborne parasites (FBP) are of major public health importance and warrant appropriate detection and control strategies. Most of the FBP considered for risk-ranking by a panel of experts are potentially transmitted via consumption of contaminated fresh produce, including berries. In

Artículos

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PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

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Protocolos

KiCqStart™ Probe qPCR ReadyMix™

KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.

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