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Merck

I9640

Sigma-Aldrich

IgG from bovine serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Sinónimos:

Bovine IgG

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

conjugado

unconjugated

Nivel de calidad

grado

technical grade

Análisis

≥80% (SDS-PAGE)

formulario

buffered aqueous solution

técnicas

cell culture | mammalian: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Bovine immunoglobulin, protein source obtained from immunoglobulin derived from serum, is a naturally occurring bioactive proteins and peptides. Fetal calf or bovine serum IgG can be depleted with protein G affinity chromatography and various concentration of this product can be used in RPMI-1640 medium to produce mouse hybridoma cell line CAy-G (anti-HBs IgG1 mAb producing hybridoma cell). The heat aggregated bovine serum IgG is also useful in down regulating mannose receptor expression. IgG from bovine serum reacts specifically with mouse.

Inmunógeno

IgG is isolated from pooled bovine serum.

Aplicación

IgG from bovine serum is used as a supplement in cell culture.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.2 with 15 mM sodium azide as preservative

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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S Schreiber et al.
Journal of immunology (Baltimore, Md. : 1950), 147(4), 1377-1382 (1991-08-15)
We previously described the presence of an inhibitory protein contained in the 20 to 40% (NH4)2SO4 precipitable fraction of FCS that down-regulates expression of mannose receptors on bone marrow-derived macrophages. We now identify aggregated bovine IgG as the main inhibitory
C Aybay et al.
Journal of immunological methods, 233(1-2), 77-81 (2000-01-29)
Fetal calf serum (FCS) was depleted of its immunoglobulin G (IgG) in a rapid procedure using protein G affinity chromatography. 20 ml of FCS was depleted of its IgG in less than 80 min by applying 5 ml of FCS
Manita Shakya et al.
Endocrinology (2021-03-12)
Pro-opiomelanocortin (POMC) neurons form an integral part of the central melanocortin system regulating food intake and energy expenditure. Genetic and pharmacological studies have revealed that defects in POMC synthesis, processing, and receptor signaling lead to obesity. It is well established
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.

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