I4753

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

• Sinónimos: β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase
• Número de CAS: 9001-57-4
• Comisión internacional de enzimas: 3.2.1.26 (BRENDA, IUBMB)
• Número CE: 232-615-7
• Número MDL: MFCD00131410
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Grade X
• formulario: solid
• actividad específica: ≥300 units/mg solid
• mol peso: ~260 kDa
• actividad extraña: α-galactosidase (melibiase), essentially free
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

Aplicación

Invertase from Candida utilis has been used:

• as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
• to determine the sucrose content in ethanolic extract of leaf tissues
• to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Acciones bioquímicas o fisiológicas

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

Propiedades físicas

Michaelis constant : 1.5x10 ^-2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70o^C
pH Stability : pH 4.0 - 6.0 (50o^C, 10min)
Thermal stability : below 60o^C (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6⁾

Definición de unidad

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

Nota de preparación

Chromatographically purified

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)