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Merck

I2011

Sigma-Aldrich

Anti-Human IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:

Anti-IgG antibody, Rabbit anti-Human IgG

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

IgG fraction of antiserum

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

técnicas

indirect ELISA: 1:80,000
quantitative precipitin assay: 3.0-4.0 mg/mL

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.

Especificidad

The antiserum is determined to be immunospecific for human IgG. Reactivity with light chains is observed.

Aplicación

Anti-Human IgG (whole molecule) antibody produced in rabbit has been used in immunoprecipitation and enzyme linked immuno sorbent assay (ELISA).
Anti-Human IgG (whole molecule) antibody produced in rabbit was coated on Sepharose beads to immunoprecipitate IgG from HUVEC cultured conditioned medium.

Acciones bioquímicas o fisiológicas

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is notrecommended.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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D Carvalho et al.
The Journal of clinical investigation, 97(1), 111-119 (1996-01-01)
IgG autoantibodies that bind human endothelial cells (AECA) were detected by ELISA in 30 of 42 samples of sera from patients with scleroderma. Pretreatment of human umbilical vein endothelial cells with AECA-positive scleroderma sera, or IgG purified from these sera
Molecular Genetics of Immunoglobulin (1987)
Adherence of platelets to in situ albumin-binding surfaces under flow conditions: role of surface-adsorbed albumin
Thakurta SG, et al.
Biomedical Materials (Bristol, England), 7(4), 045007-045007 (2012)
J Hamm et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(24), 12839-12844 (1997-12-16)
The anti-idiotype approach is based on the assumption that an antibody specific for a receptor-binding domain of a ligand could be structurally related to the receptor. Therefore, a structural mimic of a receptor-binding domain, selected with an anti-ligand antibody, might
Wei Wang et al.
Journal of pharmaceutical sciences, 96(1), 1-26 (2006-09-26)
The number of therapeutic monoclonal antibody in development has increased tremendously over the last several years and this trend continues. At present there are more than 23 approved antibodies on the US market and an estimated 200 or more are

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