H6287

Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal

clone HDAC1-21, purified from hybridoma cell culture

• Sinónimos: Monoclonal Anti-Histone Deacetylase 1 (HDAC1) antibody produced in mouse
• Número MDL: MFCD01633420
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: HDAC1-21, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~65 kDa
• reactividad de especies: mouse, human
• concentración: ~2 mg/mL
• técnicas: immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using total cell extracts of HeLa cells
• isotipo: IgG3
• Nº de acceso UniProt: Q13547
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HDAC1(3065) ; mouse ... Hdac1(433759)

Descripción

Descripción general

Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the HDAC1-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human and mouse histone deacetylase 1 (HDAC1).

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.

Aplicación

Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunoblotting

Acciones bioquímicas o fisiológicas

HDAC1 is widely studied and is shown to actively modulate the eukaryotic chromatin structure. It deacetylyses lysine residues on core histones (H2A, H2B, H3 and H4) at the N-terminal. It is a vital component of cofactor complexes and is involved in transcription regulation. Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing.

Forma física

solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids