GE17-5319-01
HisTrap™ Fast Flow
Cytiva 17-5319-01, pack of 5 × 1 mL
About This Item
Productos recomendados
envase
pack of 5 × 1 mL
fabricante / nombre comercial
Cytiva 17-5319-01
Parámetros
<20 mL/min flow rate
42 psi
lecho tamaño
16 mm × 25 mm
lecho volumen
5 mL
columna D.I.
16 mm
Matriz
6% cross-linked agarose
tamaño de partícula
45-165 μm
diámetro promedio
90 μm
cleaning
2-14(Ni2+-stripped medium.)
Intervalo de trabajo
3-12(Ni2+-stripped medium.)
capacidad
~40 mg binding capacity(histidine-tagged protein)
idoneidad
suitable for bioprocess medium
Descripción general
Ni Sepharose™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
The high flow rates made possible by the Ni Sepharose™ 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Características y beneficios
- High binding capacity, approx. 40 mg/mL medium.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Almacenamiento y estabilidad
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.
Si necesita más asistencia, póngase en contacto con Atención al cliente
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Artículos
This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
Protocolos
This page shows how to perform a cell disruption und prepare membranes with products from GE Healthcare.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
Contenido relacionado
Purification methods for downstream analysis reduce sample complexity, remove interferences, and concentrate analytes for more accurate results.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico