GE17-0969-01
IgG Sepharose™ 6 Fast Flow
Cytiva 17-0969-01, pack of 10 mL
Sinónimos:
IgG Column
About This Item
Productos recomendados
ligand
human IgG
envase
pack of 10 mL
fabricante / nombre comercial
Cytiva 17-0969-01
condiciones de almacenamiento
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
Matriz
highly cross-linked 6% agarose
diámetro promedio
90 μm
cleaning in place
3-12
Intervalo de trabajo
3-10
capacidad
~3 mg binding capacity(Protein A)
temp. de almacenamiento
2-8°C
Descripción general
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
Características y beneficios
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
Almacenamiento y estabilidad
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
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Certificados de análisis (COA)
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Artículos
This page shows how to perform a separation with IgG Sepharose 6 Fast Flow from GE Healthcare, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocolos
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
This page shows how to prepare samples for purification with affinity chromatography.
Contenido relacionado
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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