GE17-0921-07
IMAC Sepharose™ 6 Fast Flow
Cytiva 17-0921-07, pack of 25 mL
About This Item
Productos recomendados
envase
pack of 25 mL
fabricante / nombre comercial
Cytiva 17-0921-07
Matriz
6% cross-linked agarose
tamaño de partícula
45-165 μm
diámetro promedio
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
Intervalo de trabajo
3-12
capacidad
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
idoneidad
suitable for bioprocess medium
Descripción general
Aplicación
IMAC Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support
Características y beneficios
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
Almacenamiento y estabilidad
Nota de análisis
Información legal
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
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Artículos
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Protocolos
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.
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