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Key Documents

GE17-0034-02

Sephadex® G-25 Coarse

Cytiva 17-0034-02, pack of 500 g

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About This Item

Código UNSPSC:
41115722
NACRES:
NA.56

envase

pack of 500 g

fabricante / nombre comercial

Cytiva 17-0034-02

condiciones de almacenamiento

dry media

matriz

cross-linked dextran

tamaño de partícula

>100 μm (dry)

cleaning in place

2-13

working range

2-13

idoneidad

suitable for bioprocess medium

Descripción general

Sephadex® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características y beneficios

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Excellent recovery and minimum sample dilution
  • Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
  • BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Código de clase de almacenamiento

13 - Non Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

Fractional Precipitation Protocol for Affinity Chromatography Samples
Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico