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Merck

G9422

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

Sinónimos:

BGP, Glycerol 2-phosphate disodium salt hydrate

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About This Item

Fórmula lineal:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
Número de CAS:
Peso molecular:
216.04 (anhydrous basis)
Número CE:
Número MDL:
Código UNSPSC:
12352201
ID de la sustancia en PubChem:
NACRES:
NA.25

origen biológico

synthetic (chemical)

Nivel de calidad

Línea del producto

BioUltra

Análisis

≥99% (titration)

formulario

powder

técnicas

cell culture | mammalian: suitable
cell culture | plant: suitable

impurezas

≤0.1% Insoluble matter
≤0.5 mol % L-α-isomer

color

white

mp

102-104 °C (lit.)

solubilidad

water: soluble

trazas de anión

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

trazas de catión

Al: ≤0.001%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.001%
K: ≤0.005%
Mg: ≤0.0005%
Pb: ≤0.001%
Zn: ≤0.0005%

cadena SMILES

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

Clave InChI

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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Descripción general

Glycerophosphoric acid disodium salt hydrate, also known as BGP, is a versatile compound with applications across various research domains, including cell biology, biochemical research, and metabolomics. Recognized as an endogenous metabolite and a serine-threonine phosphatase inhibitor, BGP is extensively utilized in chitosan hydrogel formulations for drug delivery research. It serves as a phosphate source for cell growth and recombinant protein production, offering advantages in media formulations by reducing precipitation at higher pH levels. Additionally, BGP finds applications in micro-arc oxidation electrolyte solutions, contributing to the creation of photocatalytic coatings.
In cell biology, β-Glycerophosphate disodium salt plays a pivotal role as a phosphate group donor in matrix mineralization studies and accelerates calcification in vascular smooth muscle cells. It also promotes bone matrix mineralization when delivered to osteoblasts, providing a crucial source of phosphate ions. In the realm of biochemical research, BGP functions as a classical serine-threonine phosphatase inhibitor in kinase reaction buffers, offering broad-spectrum inhibition. Its role as an organic phosphate donor is particularly valuable in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. Moreover, BGP is utilized to buffer M17 media for Lactococcus culture in recombinant protein expression. The comprehensive utility of BGP spans multiple areas of research, making it an essential component in studies encompassing cell biology, biochemical research, and metabolomics.

Aplicación

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Características y beneficios

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Cell culture and Plant cell culture
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • BioUltra Grade for your Cell Biology and Biochemical research

Calidad

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Otras notas

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Pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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J Douglas et al.
The Journal of applied bacteriology, 56(2), 321-326 (1984-04-01)
An easily prepared medium, originally designed for the cultivation of lactic phages, has been found to have much wider application. Experience in its use over a ten year period with a range of physiologically diverse bacteria, for teaching and research
Maryam Iravani et al.
Endocrine connections, 8(9), 1302-1309 (2019-08-23)
Estrogens may affect bone growth locally or systemically via the known estrogen receptors ESR1, ESR2 and G protein-coupled estrogen receptor 1 (GPER1). Mouse and human growth plate chondrocytes have been demonstrated to express GPER1 and ablation of this receptor increased
A Herrera et al.
Materials science & engineering. C, Materials for biological applications, 103, 109760-109760 (2019-07-28)
In the design of macroporous biomaterial scaffolds, attention is payed predominantly to the readily accessible macroscopic mechanical properties rather than to the mechanical properties experienced by the cells adhering to the material. However, the direct cell mechanical environment has been
Lei Zhou et al.
Life sciences, 212, 129-137 (2018-10-06)
The exact mechanism underlying osteoblast differentiation and proliferation remains to be further elucidated. The circadian clock has been universally acknowledged controls behavioral activities and biological process in mammals. Cryptochrome 1 (Cry1), one of the core circadian genes, is associated with
Lucas R Brun et al.
SpringerPlus, 3, 676-676 (2014-12-19)
The aim of this study was to evaluate the effect of strontium ranelate (SrR) on bone mineral density (BMD) and bone turnover markers after 1 year of treatment. Additionally, the effect of SrR in bisphosphonate-naïve patients (BP-naïve) compared to patients previously

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