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Key Documents

G7509

Sigma-Aldrich

GABase from Pseudomonas fluorescens

lyophilized powder, Protein ≥30 % by biuret

Sinónimos:

GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase

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About This Item

Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32

origen biológico

Pseudomonas fluorescens

Nivel de calidad

Análisis

≥30  % (biuret)

formulario

lyophilized powder

actividad específica

≥0.5 units/mg protein

composición

Protein, ≥30% biuret

técnicas

inhibition assay: suitable

temp. de almacenamiento

−20°C

Descripción general

Research area: Neuroscience


GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.

Aplicación

GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).

Acciones bioquímicas o fisiológicas

GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.

Definición de unidad

One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.

Forma física

Partially purified lyophilized powder containing buffer salts and stabilizer

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that under acidic conditions extracellular glutamate
A Vidal-Cros et al.
The Biochemical journal, 229(3), 675-678 (1985-08-01)
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis
Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
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Plant, cell & environment, 38(3), 600-613 (2014-07-31)
γ-Aminobutyric acid (GABA) accumulates in many plant species in response to environmental stress. However, the physiological function of GABA or its metabolic pathway (GABA shunt) in plants remains largely unclear. Here, the genes, including glutamate decarboxylases (SlGADs), GABA transaminases (SlGABA-Ts) and
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Isolation and functional characterization of microorganisms are relevant steps for generating starter cultures with functional properties, and more recently, those related to improving mental health. Milk kefir grains have been recently investigated as a source of health-related strains. This study

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