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G1N10

Sigma-Aldrich

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Sinónimos:

Mammalian Genomic DNA Miniprep, Gen Elute

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About This Item

Código UNSPSC:
41105501
NACRES:
NA.55

uso

sufficient for 10 purifications

temp. de almacenamiento

15-25°C

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Descripción general

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Aplicación

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Características y beneficios

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principio

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Otras notas

For additional information, please see www.sigma-aldrich.com/genomicdna.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

Opcional

Referencia del producto
Descripción
Precios

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Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

483.8 °F

Punto de inflamabilidad (°C)

251 °C

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Certificados de análisis (COA)

Lot/Batch Number
SLCL7191
SLCK8229
SLCF6078
SLCC7185
SLCB3846

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