G0290

Anti-GATA1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-ERYF1, Anti-Erythroid transcription factor 1, Anti-Globin transcription factor 1
• Número MDL: MFCD02096392
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 45 kDa
• reactividad de especies: human
• envase: antibody small pack of 25 μL
• técnicas: microarray: suitable; western blot: 1:200 using a whole extract from a human chronic myelogenous leukemia cell line, K562
• Nº de acceso UniProt: P15976
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GATA1(2623)

Descripción

Descripción general

GATA is an important cis-element factor in the haematopoietic lineage belongs to GATA-binding protein family. It was first identified in the analysis of globin gene promoters and enhancers. There are different types of GATA proteins: GATA-1, GATA-2, GATA-3. It is expressed in erythroid, mast, megakaryocytic lineages, multipotential progenitor cells and lines.

GATA-1 (ERYF1, GF-1, NF-1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells.

Inmunógeno

synthetic peptide corresponding to the C-terminal region of human GATA-1 (amino acids 394-413).

Aplicación

Anti-GATA1 antibody produced in rabbit has been used in gel mobility shift assay and DNase I footprinting. It has also been used in confocal microscopy analysis.

Acciones bioquímicas o fisiológicas

GATA is a lineage-restricted transcription factor which plays a pivotal role in erythroid differentiation. It possesses potential binding sites in the regulatory sequences of all erythroid genes. GATA is expressed at higher levels during erythroid maturation. It is essential for terminal differentiation of erythrocytes and megakaryocytes and associated in vivo with the acetyl transferase p300/CBP. Mutation in GATA1 causes thrombocytopenia and abnormal platelet function.

GATA-binding factor 1 (GATA-1) is involved in blocking apoptosis of precursor cells and in controlling the balance between proliferation and cell cycle arrest.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)