F5392

Fibroblast Growth Factor-Basic from bovine pituitary

FGF-Basic, suitable for cell culture

• Sinónimos: FGF2, bFGF
• Número de CAS: 106096-93-9
• Número MDL: MFCD00081382
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: bovine pituitary glands
• Nivel de calidad: 200
• Análisis: ~90% (SDS-PAGE and N-terminal analysis)
• formulario: lyophilized powder
• potencia: 0.03-3.0 ng/mL ED₅₀/EC₅₀
• calidad: endotoxin tested
• mol peso: 16.4 kDa
• envase: pkg of 1 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.5 EU/vial
• color: white
• Nº de acceso UniProt: P03969
• temp. de almacenamiento: −20°C
• Información sobre el gen: bovine ... FGF2(281161)

Descripción

Descripción general

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Aplicación

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
• In the generation of directly reprogramming fibroblast
• In human glioma cell culture

Acciones bioquímicas o fisiológicas

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Forma física

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Nota de preparación

Purified glands by a modification of the method of Gospodarowicz, et al.

Nota de análisis

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)