E0127
Elastase from porcine pancreas
Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein
Sinónimos:
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Productos recomendados
tpo
Type III
Nivel de calidad
formulario
lyophilized powder
actividad específica
≥4.0 units/mg protein
mol peso
25.9 kDa
composición
Protein, 55-85%
solubilidad
200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear
temp. de almacenamiento
−20°C
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Aplicación
Elastase from porcine pancreas has been used in a study to identify a novel bone/calcium metabolism-regulating factor in porcine pancreas. Elastase from porcine pancreas has also been used in a study to investigate the effect of a specific synthetic inhibitor of neutrophil elastase (ONO-5046) on the course of acute hemorrhagic pancreatitis in dogs.
Elastase from porcine pancreas has been used in the proteolytic cleavage of UMP synthase (Uridine-5′-monophosphate synthase). It has also been used along with collagenase to disperse tissue into single cells from the thoracic media, abdominal aortic media and intima of rabbit.
Acciones bioquímicas o fisiológicas
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides, p-dinitrophenyl diethylphosphate and high salt concentrations. Elastase is extensively used in tissue and cell dissociation procedures. It is effective in the isolation of Type II lung cells.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Envase
Package size based on protein content
Calidad
Contains trypsin activity
Definición de unidad
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Forma física
Contains sodium carbonate.
Nota de preparación
2x crystallized and chromatographically purified
Aplicación
Referencia del producto
Descripción
Precios
inhibidor
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
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