D8312
REDTaq® Genomic DNA Polymerase
with MgCl2
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About This Item
Productos recomendados
Nivel de calidad
formulario
liquid
uso
sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 2500 reactions
Características
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
concentración
1 unit/μL
técnicas
PCR: suitable
color
red
entrada
purified DNA
aplicaciones
agriculture
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
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Descripción general
REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.
Aplicación
REDTaq® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA.
Características y beneficios
- Enhanced amplification on genomic and difficult DNA templates
- Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
- Quick recognition and confirmation of appropriate mixing
- No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
- The samples can be re-amplified as in nested PCR
- The red dye migrates faster than bromophenol blue
- Greater consistency across reactions due to proper mixing
Definición de unidad
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.
Información legal
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- P219210X PCR BufferSDS
Producto relacionado
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Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Protocolos
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Method for PCR using genomic DNA or other complex templates
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