CWA-1006

Anti-Mouse CD11b (M1/70) ColorWheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: M1/70, monoclonal
• Línea del producto: ColorWheel^®
• formulario: lyophilized
• mol peso: calculated mol wt 127.48 kDa
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): rat, human
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnicas: flow cytometry: suitable
• isotipo: IgG2bκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: ABK96806.1
• Nº de acceso UniProt: P05555
• compatibilidad: for use with ColorWheel^® Dyes (Required, sold separately)
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Itgam(16409)

Descripción

Descripción general

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidad

Clone M1/70 is a rat monoclonal antibody that detects murine CD11b.

Inmunógeno

Mouse spleen cells.

Aplicación

Quality Control Testing:

Evaluated by Flow Cytometry in mouse bone marrow cells.

Flow Cytometry Analysis (FC): Staining of one million mouse bone marrow cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1006, Anti-CD11b Antibody (M1/70) ColorWheel^® Rat mAb and Cat. No. CWD-PE ColorWheel^® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilidad

Requires ColorWheel^® Dye (Sold Separately)

Descripción de destino

Integrin alpha-M (UniProt: P05555; also known as CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CD11b) is encoded by the Itgam gene (Gene ID: 16409) in murine species. Integrins are alpha/beta heterodimeric adhesion receptors that couple the extracellular matrix on other cells with the contractile cytoskeleton to transduce mechanochemical signals across the plasma membrane. CD11b is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-16), which is cleaved off in the mature form that contains a long extracellular domain (aa 17-1105), a transmembrane domain (aa 1106-1129), and a short cytoplasmic domain (aa 1130-1153). It is predominantly expressed in monocytes and granulocytes. It is usually found as a heterodimer of alpha and beta subunits and associates with Integrin beta-2 (ITGB2). It has been implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It contains a metal ion-dependent adhesion site (MIDAS) in the integrin A domain, which is essential for stabilization of domain A in high affinity state. The major ligand recognition site of CD11b/CD18 has been mapped to the A-domain in the CD11b subunit (CD11bA). Both magnesium and calcium ions exhibit equal affinities for the inactive CD11bA, however, upon activation there is a 10-fold increase in the binding affinity of magnesium ions without affecting affinity of calcium ions. Two isoforms of CD11b have been reported that are produced by alternative splicing.

Forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitución

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Información legal

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable