CRISPR17

CRISPR Activator Human Oct4 Positive Control Plasmid

gRNA Only Expression Plasmid

• Código UNSPSC: 41106609
• NACRES: NE.02

Propiedades

• Nivel de calidad: 200
• formulario: liquid
• envase: vial of 50 μL
• concentración: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• aplicaciones: CRISPR
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

Aplicación

Functional Genomics/Target Validation

• Epigenetic Modification
• Transcriptional Activation

Características y beneficios

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

Componentes

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).

Ligadura / enlace

To order U6-gRNA plasmids, please visit sigma.com/crisprs

Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.

Otras notas

Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.

Información legal

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable