CG200
cGMP Enzyme Immunoassay Kit, Direct
sufficient for 96 assays
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Productos recomendados
uso
sufficient for 96 assays
Nivel de calidad
técnicas
ELISA: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... PRKG1(5592) , PRKG2(5593)
Aplicación
Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.
Solo componentes del kit
Referencia del producto
Descripción
- 0.1 M Hydrochloride Acid 1 x 30
- Acetic Anhydride 1 x 1
- cGMP-Alkaline Phosphatase Conjugate 1 x 5
- cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
- Cyclic GMP Standard 1 x 0.5
- Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
- Neutralizing Reagent 1 x 6
- p-Nitrophenyl Phosphate Substrate Solution 1 x 20
- Plate Sealer 1 ea
- Stop Solution 1 x 5
- Triethylamine 1 x 2
- Wash Buffer Concentrate 1 x 30
Ver todo (12)
Palabra de señalización
Danger
Frases de peligro
Clasificaciones de peligro
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Órganos de actuación
Respiratory system
Riesgos supl.
Código de clase de almacenamiento
3 - Flammable liquids
Punto de inflamabilidad (°F)
120.2 °F
Punto de inflamabilidad (°C)
49 °C
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Chard, T.
An Introduction to Radioimmunoassay and Related Techniques (1990)
Tijssen, P.
Practice and Theory of Enzyme Immunoassays (1985)
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