SHP-77

98110201, human lung, Not specified

• Sinónimos: Human SHP-77, SHP-77 Cell Line
• Código UNSPSC: 41106514

Propiedades

• product name: SHP-77, 98110201
• origen biológico: human lung
• modo de crecimiento: Adherent/aggregates in suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian lung small cell carcinoma, large cell variant

Descripción línea celular

SHP-77 was established in 1977 being derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung. The patient was a 54 year old male with blood type O, Rh+. The cell line has a variety of unusual characteristics including the morphology of an undifferentiated large cell variant of small cell lung carcinoma (SCLC) but biochemical properties of a classical SCLC. SHP-77 express neural cell adhesion molecule (NCAM), NHK-1, CD56 and the cell surface leucocyte antigen Leu-7(HNK-1;CD57). Other neuroendocrine markers are L-dopa decarboxylase and dense core secretory granules. Gland formation and intracytoplasmic lamellar bodies have been shown by electron microscopy. This cell line is useful as in vitro target in 51Cr and III In release cytotoxicity assays. They are suitable for in vitro nude mice assays for evaluation of immune reactivity of cells and serum from lung cancer patients. In addition, they serve to evaluate the immune status of patients with SCLC who received radiation or chemotherapy treatment. SHP-77 cells are tumorigenic in nude mice and grow as circumscribed nodules without metastases formation. They have a reported doubling time of 96 hours.

Aplicación

SCLC studies, cytotoxicity assays, immunological studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS).

Rutina de subcultivo

Maintain cultures between 4-9 x 100,000 cells / ml; 5% CO₂; 37°C. Cells grow as mixture of loosely adherent and floating clusters.

Otras notas

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