293-PERV-PK-CIRCE

97051411, human kidney, Not specified

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: 293-PERV-PK-CIRCE, 97051411
• origen biológico: human kidney
• modo de crecimiento: Adherent aggregates
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human embryo kidney, infected with endogenous retrovirus of pigs

Descripción línea celular

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

Aplicación

Pig retrovirus studies, infectivity assays

Medio de cultivo

DMEM + 2mM Glutamine + 10% HI-FBS

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

Otras notas

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