COR-L 23/CPR

96042336, human lung, Not specified

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: COR-L 23/CPR, 96042336
• origen biológico: human lung
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian lung, large cell carcinoma

Descripción línea celular

The cell line COR-L23/CPR is a drug-resistant variant of COR-L23 (Sigma Catalogue number. 92031919). The line was developed by growing the parent line in increasing concentrations of cisplatin. Cisplatin accumulation is reduced in COR-L23/CPR and cross resistance to melphalan and other platinum compounds has been found. Cells tend to grow in clumps.

Aplicación

Analysis of acquired drug resistance, study of large cell carcinoma of the lung

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8
vWA: 19

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 0.05μg / ml Cisplatin + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5 x 10,000 cells / cm² using 0.25 % trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.

Otras notas

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