MOR/CPR

96042333, human lung, Not specified

• Código UNSPSC: 41106514
• origen biológico: human lung
• modo de crecimiento: Aggregates in suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• enfermedades relevantes: cancer

Propiedades

• Nombre del producto: MOR/CPR, 96042333
• origen biológico: human lung
• modo de crecimiento: Aggregates in suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human lung adenocarcinoma, drug-resistant

Descripción línea celular

MOR/CPR has been developed by growing the parent line, MOR, in increasing concentrations of cisplatin. The cells are cross-resistant to melphalan but show little or no cross-resistance to other platinum compounds. Cisplatin accumulation has been found to be reduced compared to the parent line.

Aplicación

Analysis of acquired drug resistance

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 1μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Otras notas

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