TGP 55

95062833, mouse pancreas, Polymorph

• Código UNSPSC: 41106514

Propiedades

• product name: TGP 55, 95062833
• origen biológico: mouse pancreas
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Polymorph
• productos: Somatostatin and Insulin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse pancreatic small cell anaplastic carcinoma

Descripción línea celular

TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.

Aplicación

Study of exocrine pancreas carcinomas, hormone secretion studies.

Medio de cultivo

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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