AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• Código UNSPSC: 41106514

Propiedades

• origen biológico: rat pancreas
• envase: tube of 5 μg 93100618-DNA-5UG; pkg of vial of cells 93100618-1VL
• modo de crecimiento: Semi-adherent aggregates
• cariotipo: Not specified
• morfología: Pancreas cells
• productos: Amylase and exocrine enzymes
• receptores: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat exocrine pancreatic tumour

Descripción línea celular

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Aplicación

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

Otras notas

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