DMS 153

95062827, human lung (liver metastasis), Not specified

• Código UNSPSC: 41106514

Propiedades

• product name: DMS 153, 95062827
• origen biológico: human lung (liver metastasis)
• modo de crecimiento: Semi-adherent aggregates
• cariotipo: Modal no. 41-42
• morfología: Not specified
• productos: Not specified
• receptores: Bombesin, Epidermal growth factor (EGF)
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian lung small cell carcinoma, metastasis to liver

Descripción línea celular

DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

Aplicación

Human lung tumour model

Perfil del ADN

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15

Medio de cultivo

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.Cells grow in large clumps.

Otras notas

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