BE(2)-C

95011817, human nerve, Neuroblast-like

• Código UNSPSC: 41106514

Propiedades

• product name: BE(2)-C, 95011817
• origen biológico: human nerve
• modo de crecimiento: Adherent
• cariotipo: Modal no. 44
• morfología: Neuroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian neuroblastoma

Descripción línea celular

BE(2)-C is a clonal sub-line of SK-N-BE(2) (ECCAC catalogue no. 95011815) which was isolated from bone marrow of a 22-month-old male with disseminated neuroblastoma in 1972. They are reported to be multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine. The cells show a small, refractile morphology with short, neurite-like cell processes and tend to grow in aggregates.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicación

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 11
vWA: 18

Medio de cultivo

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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