AT6.1

94101452, rat prostate, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: AT6.1, 94101452
• origen biológico: rat prostate
• modo de crecimiento: Adherent
• cariotipo: 44XY, +4, + 12 del(3)(3q32-36)del(15)p14
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat dorsal prostatic adenocarcinoma

Descripción línea celular

A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.

Aplicación

Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localised to 11p11.2-13.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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