SK-PN-DW

94092301, human nerve, Polygonal

• Código UNSPSC: 41106514

Propiedades

• product name: SK-PN-DW, 94092301
• origen biológico: human nerve
• modo de crecimiento: Semi-adherent
• cariotipo: 82XXY
• morfología: Polygonal
• productos: Pro-cholecystokinin (Pro-cck)
• receptores: Vimentin, P53, anti-MIC2
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human primitive neuroectodermal tumour

Descripción línea celular

SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.

Aplicación

Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an in vitro system for investigation of the post translational processing of the cck preprohormone.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16

Medio de cultivo

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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