WHO NP199 human cell line

IHW Number: 9134, 94082233

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human blood
• envase: tube of 5 μg 94082233-DNA-5UG; pkg of vial of cells 94082233-1VL
• etnicidad: East Asian
• género: female
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Descripción general

Consanguineous: No

Homozygous: No

Descripción línea celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil del ADN

A: 02 30
Cw: 602
B: 13 46
HLA DRB1: 07 09
HLA DRB4: 101
HLA DQAP: 2.1 3.1
HLA DQA1: 0201 0302
HLA DQB1: 0201 0303
HLA DPB1: 501

Perfil serológico

HLA A: 2 30
HLA C: 6
HLA B: 13 46
Bw4/bw6: 6

Medio de cultivo

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutina de subcultivo

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cláusula de descargo de responsabilidad

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.