DH82

94062922, dog blood, Macrophage

• Sinónimos: DH82 Cell Line
• Código UNSPSC: 41106514

Propiedades

• product name: DH82, 94062922
• origen biológico: dog blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Macrophage
• productos: Not specified
• receptores: FC-gamma receptors
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Canine Monocyte-macrophage

Descripción línea celular

Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Maintain cultures at 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.

Otras notas

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