TGP 52

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94022429, mouse pancreas, Trabecular

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse pancreas
• envase: tube of 5 μg 94022429-DNA-5UG; pkg of vial of cells 94022429-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Trabecular
• productos: Somatostatin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse islet cell tumour

Descripción línea celular

TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.

Aplicación

Study of endocrine pancreas carcinomas, hormone assays.

Medio de cultivo

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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