TGP 49

94022427, mouse pancreas, Trabecular

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: TGP 49, 94022427
• origen biológico: mouse pancreas
• envase: tube of 5 μg 94022427-DNA-5UG; pkg of vial of cells 94022427-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Trabecular
• productos: Somatostatin (low levels)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse pancreatic acinar carcinoma

Descripción línea celular

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

Aplicación

Study of endocrine pancreas carcinomas, hormone assays.

Medio de cultivo

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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