DBTRG.05MG

93061119, human brain, Glial

• Código UNSPSC: 41106514

Propiedades

• product name: DBTRG.05MG, 93061119
• origen biológico: human brain
• modo de crecimiento: Adherent
• cariotipo: 2n = 87-91, hypotetraploid
• morfología: Glial
• productos: Not specified
• receptores: Epidermal growth factor (EGF) R
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human glioblastoma

Descripción línea celular

Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.

Aplicación

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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