EJG

93051803, bovine adrenal gland, Endothelial

• Código UNSPSC: 41106514

Propiedades

• product name: EJG, 93051803
• origen biológico: bovine adrenal gland
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Endothelial
• productos: Factor VIII
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Bovine adrenal medulla endothelial

Descripción línea celular

The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.

Aplicación

Blood clotting studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Passage cells every 5 to 7 days.

Otras notas

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