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AB3565

Sigma-Aldrich

Anti-PARP (214/215) cleavage site Antibody

Chemicon®, from rabbit

Sinónimos:

Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, ARTD1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

mouse, bovine, rat, human

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
western blot: suitable

Nº de acceso NCBI

Descripción general

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Especificidad

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.
The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Inmunógeno

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

Aplicación

Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.
For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

Calidad

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

Ligadura / enlace

Replaces: 04-575; 04-576

Forma física

Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
ImmunoAffinity Purified

Almacenamiento y estabilidad

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Nota de análisis

Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis
Kaufmann, S H, et al
Cancer Research, 53, 3976-3985 (1993)
J C Leemans et al.
Journal of immunology (Baltimore, Md. : 1950), 166(7), 4604-4611 (2001-03-20)
Mycobacterium tuberculosis bacilli are intracellular organisms that reside in phagosomes of alveolar macrophages (AMs). To determine the in vivo role of AM depletion in host defense against M. tuberculosis infection, mice with pulmonary tuberculosis induced by intranasal administration of virulent
Model of inhibition of the NPM-ALK kinase activity by herbimycin A.
Turturro, F. et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 8(1), 240-245 null
Poly(ADP-ribose) polymerase-1 (PARP-1) contributes to the barrier function of a vertebrate chromatin insulator.
Aker, M; Bomsztyk, K; Emery, DW
The Journal of Biological Chemistry null
Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
Talavera-Adame, D; Gupta, A; Kurtovic, S; Chaiboonma, KL; Arumugaswami, V; Dafoe, DC
Stem Cells and Development null

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