293 N3S

92052131, human kidney, Not specified

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: 293 N3S, 92052131
• origen biológico: human kidney
• envase: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Adenovirus 5 early region (E1) antigens
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human embryo kidney

Descripción línea celular

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Medio de cultivo

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

Rutina de subcultivo

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

Otras notas

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