HT29 gluc C1

92012401, human colon, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: HT29 gluc C1, 92012401
• origen biológico: human colon
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian colon adenocarcinoma

Descripción línea celular

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

Medio de cultivo

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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