NCI-H292

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091815, human lung, Epithelial

• Sinónimos: H292 Cells, NCI-HUT-292 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human lung
• envase: tube of 5 μg 91091815-DNA-5UG; pkg of vial of cells 91091815-1VL
• modo de crecimiento: Adherent
• cariotipo: Modal no. 47
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human lung, mucoepidermoid carcinoma

Descripción línea celular

Derived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. Cells retain mucoepidermoid characteristics in culture and support replication of Hepatitis B virus. Some of the cells contain numerous small mucin-containing granules and are L-DOPA decaroxylase negative. This cell line has been selected as a prototype for transfecting subgenome fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive and also stain positively for keratin and vimentin but negative for neurofilament triplet protein. Ethnicity: Black.

Aplicación

NCI-H292 has been used to determine the ability of the cell line to support the replication of mumps virus.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.