KG1a

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91030101, human bone marrow, Lymphoblast

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human bone marrow
• envase: tube of 5 μg 91030101-DNA-5UG; pkg of vial of cells 91030101-1VL
• modo de crecimiento: Suspension
• cariotipo: 2n = 46, pseudodiploid
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian bone marrow acute myelogenous leukaemia

Descripción línea celular

Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Medio de cultivo

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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