PC-3 Cell Line human

90112714, human prostate, Epithelial

• Sinónimos: PC.3 Cells, PC3 Cells
• Código UNSPSC: 41106514
• eCl@ss: 32190102

Propiedades

• product name: PC-3 Cell Line human, 90112714, Caucasian prostate adenocarcinoma
• origen biológico: human prostate
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, triploid, modal no. 62
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian prostate adenocarcinoma

Descripción línea celular

Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-α reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicación

PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17

Medio de cultivo

Coons Modified Ham’s F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.

Otras notas

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