GS-109-V-20

90110505, human skin, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• product name: GS-109-V-20, 90110505
• origen biológico: human skin
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian skin fibroblast Gardner′s syndrome

Descripción línea celular

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

Aplicación

Tumourigenicity studies

Medio de cultivo

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

Otras notas

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