GS-109-V-34

90110504, human skin, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: GS-109-V-34, 90110504
• origen biológico: human skin
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian skin fibroblast Gardner′s syndrome

Descripción línea celular

Derived from a 6 year old Caucasian female with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Normal female karyotype.

Aplicación

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16

Medio de cultivo

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer should form in a 25cm² in 3-4 days.

Otras notas

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