K-BALB (K-234)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse embryo
• envase: tube of 5 μg 89092101-DNA-5UG; pkg of vial of cells 89092101-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n=40
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse A31 embryo Kirsten sarcoma, transformed

Descripción línea celular

Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO₂; 37°C

Otras notas

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