A375 Cell Line human

88113005, human skin, Epithelial

• Sinónimos: A-375 Cells, A375-MEL Cells
• Código UNSPSC: 41106514

Propiedades

• product name: A375 Cell Line human, 88113005
• origen biológico: human skin
• descripción: Human malignant melanoma
• modo de crecimiento: Adherent
• cariotipo: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human malignant melanoma

Descripción línea celular

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Aplicación

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Medio de cultivo

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Otras notas

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