SW480 Cell Line human

87092801, human colon, Epithelial

• Sinónimos: SW-480 Cells, SW480E Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: SW480 Cell Line human, 87092801
• origen biológico: human colon
• descripción: Human colon adenocarcinoma
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human colon adenocarcinoma

Descripción línea celular

Derived from a grade 3-4 colon adenocarcinoma. The initial cultures contained a mixture of epithelial and bipolar cells, but subsequently epithelial cells predominated. The cells produce CEA.

Aplicación

Human SW480 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used as an in vitro model to study key cellular processes required for metastasis. The cell line is used to study the effects of the transforming growth factor-β (TGF-β) pathway and heat shock protein 90 (Hsp90) on the growth of cells.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
THO1: 8
TPOX: 11
vWA: 16

Medio de cultivo

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin; 37°C. Medium change every 4-5 days. CO₂ is not required.

Otras notas

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