SW 1116

87071006, human colon, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: SW 1116, 87071006
• origen biológico: human colon
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, hypotriploid
• morfología: Epithelial
• productos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian colon adenocarcinoma

Descripción línea celular

Derived from a grade II adenocarcinoma of the colon extending into the muscularis from a 73 year old Caucasian male. The cells exhibit brush borders and produce high levels of CEA.

Aplicación

Tumourigenicity studies

Medio de cultivo

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; CO₂ is not needed. Medium change after 4-5 days. 37°C.

Otras notas

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