Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• Código UNSPSC: 41106514
• origen biológico: mouse unknown/unspecified
• modo de crecimiento: Adherent
• cariotipo: 2n = 40
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• enfermedades relevantes: cancer

Propiedades

• Nombre del producto: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• origen biológico: mouse unknown/unspecified
• modo de crecimiento: Adherent
• cariotipo: 2n = 40
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse carcinoma

Descripción línea celular

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

Aplicación

Anti-tumour test and tumourigenicity studies

Medio de cultivo

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

Otras notas

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